Manual of the HTP database

Constrained Consensus TOPology prediction

The Constrained Consensus Topology prediction method (CCTOP) is composed by three basic steps. The first step is the prediction of signal peptides. Depending on the signal peptide prediction’s output, in the case of a positive result the signal peptide is cut before any further investigation, because most of the topology prediction methods confuse signal peptides and TMHs. Next, it makes a decision whether the investigated protein sequence codes a TMP or non-TMP. The final step is the topology prediction. CCTOP, as its name shows, utilizes several methods to perform these tasks and incorporates the results of already known topological data or bioinformatical evidences into the final topology prediction as constraints. You can find more information about these steps and the results of predictions in HTP manuscript.